Cellular Senescence, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 1896
This book describes current methods for the identification and characterization of the major hallmarks of senescent cells. Chapters focus on the high heterogeneity of the senescence phenotypes, and techniques to induce and identify specific senescence programs. Additional chapters describe cellular and mouse models in which is possible to study the complex cell and non-cell autonomous functions of senescent cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Cellular Senescence: Methods and Protocols aims to ensure successful results in the further study of this vital field.
1. Detecting Cellular Senescence in Reprogramming
Coralie Cazin,Mathieu von Joest, and Han Li
2. DNA Damage In situ Ligation followed by Proximity Ligation Assay (DI-PLA)
Alessandro Galbiati and Fabrizio d’Adda di Fagagna
3. Reactive Oxygen Species Detection in Senescent Cells
Stella Victorelli and João F. Passos
4. Cellular Identification and Quantification of Senescence-associated β-Galactosidase Activity in vivo
Bennett G. Childs, Tyler J. Bussian, and Darren J. Baker
5. Relative Human Telomere Length Quantification by Real-time PCR Vasilishina A., Kropotov A., Spivak I., and Bernadotte A 6. Assessing Functional Roles of the Senescence-associated Secretory Phenotype (SASP)Nicolas Malaquin, Véronique Tu, and Francis Rodier
7. Measuring the Inflammasome in Oncogene-induced Senescence
Irene Fernández-Duran, Núria Tarrats, Priya Hari, Juan Carlos Acosta
8. Alarmin Detection in Senescent Cells
Dong Eun Kim and Albert R. Davalos
9. IMR90 ER:RAS: A Cell Model of Oncogene-induced Senescence
Andrew J Innesand Jesús Gil
10. Genotoxic Stress-induced Senescence
Dorothy N.Y. Fanand Clemens A. Schmitt
11. A Multi-parametric Assay to Evaluate Senescent Cells
Hilah Gal, Ziv Porat, and Valery Krizhanovsky
12. A Novel Quantitative Method for the Detection of Lipofuscin, the Main Byproduct of Cellular Senescence, in Fluids
Sophia V Rizou, Konstantinos Evangelou, Vassilios Myrianthopoulos, Iordanis Mourouzis, Sophia Havaki, Aikaterini Athanasiou, Panagiotis V.S. Vasileiou, Aggelos Margetis, Athanassios Kotsinas, Nikolaos G. Kastrinakis, Petros Sfikakis, Paul Townsend, Emmanuel Mikros, Constantinos Pantos, and Vassilis G. Gorgoulis
13. Measurement of Metabolite Changes in Senescent Cells by Mass Spectrometry
Christopher D. Wiley, Sonnet Davis, and Arvind Ramanathan
14. Quantification of Autophagy during Senescence
Joon Tae Park, Young-Sam Lee, and Sang Chul Park
15. Quantification of Aneuploidy in Mammalian Systems
Hilda van den Bos,Bjorn Bakker, Aaron Taudt, Victor Guryev, Maria Colomé-Tatché, Peter M. Lansdorp, Floris Foijer, and Diana C.J. Spierings
16. Identification of Genomic Alterations Through Multi-Level DNA Structural Analysis
Ryan K. Shultzaberger and John Dresios
17. Mouse Models of Accelerated Cellular Senescence
Matthew J. Yousefzadeh, Kendra Melos, Luise Angelini, Christin E. Burd, Paul D. Robbins, and Laura J. Niedernhofer
18. Methods to Quantify the NF-kB Pathway during Senescence
Lei Zhang, Jing Zhao, Aditi Gurkar, Laura J. Niedernhofer, and Paul D. Robbins
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 11-2018
Ouvrage de 253 p.
17.8x25.4 cm
