Receptor and Ion Channel Detection in the Brain, 1st ed. 2016 Methods and Protocols Neuromethods Series, Vol. 110

Preface.- Production of high-quality antibodies for the study of receptors and ion channels in brain.- Co-immunoprecipitation from brain.- Sub-synaptic membrane fractionation.- Investigation of  neurotransmitter receptors in brain slices using cell surface biotinylation.- Single nanoparticle tracking of surface ion channels and receptors in brain cells.- Radioligand binding detection of receptors in brain membranes.- Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the Brain.- Fluorescent ligands and TR-FRET to study receptor-receptor interactions in the brain.- In situ Proximity Ligation Assay to study and understand the distribution and balance of GPCR homo- and heteroreceptor complexes in the brain.- Fluorescent in situ hybridization for sensitive and specific labeling.- Autoradiographic visualization of G protein-coupled receptors in brain.- Analysis of the expression profile and regional distribution of neurotransmitter receptors and ion channels in the central nervous system using histoblots.- Immunohistochemistry for ion channels and their interacting molecules: Tips for improving antibody accessibility.- Localization of GFP-tagged proteins at the electron microscope.- Pre-embedding methods in the localization of receptors and ion channels.- Post-embedding immunohistochemistry in the localization of receptors and ion channels.- High-Resolution localization of membrane proteins by SDS-digested freeze-fracture replica labelling (SDS-FRL).- Application of virus vectors for anterograde tract-tracing and single-neuron labeling studies.- Analysis of synaptic connections using viral vectors at the electron microscopic level.- Morphological and neurochemical characterization of electrophysiologically identified cells.- Using electrophysiology to study synaptic and extrasynaptic ionotropic receptors in hippocampal neurons.- Biophysical methods to analyze direct G-protein regulation of neuronal voltage-gated calcium channels.-Electrophysiological recordings in behaving animals.- Voltammetry in behaving animals.- In vivo brain microdialysis of monoamines.- Calcium transients in single dendrites and spines of pyramidal neurons in vitro.- Dynamic recording of membrane potential from hippocampal neurons by using a FRET-based voltage biosensor.- Determination of GPCR-mediated cAMP accumulation in rat striatal synaptosomes.- GPCR-mediated MAPK/ERK cascade activation in brain slices.- Index.