Cancer Cell Signaling (3^rd Ed., 3rd ed. 2021) Methods and Protocols Methods in Molecular Biology Series, Vol. 2174

Part I: Cancer Resistance

1. Fighting Cancer Resistance: An Overview

            Martha Robles-Flores

2. Prostate Cancer Spheroids: A Three-Dimensional Model for Studying Tumor Heterogeneity

            Mauricio Rodríguez-Dorantes, Carlos David Cruz-Hernández, Sergio Alberto Cortés-Ramírez, Jenie Marian Cruz-Burgos, Juan Pablo Reyes-Grajeda, Oscar Peralta-Zaragoza, and Alberto Losada-García

3. Enrichment and Transcriptional Characterization of Stem Cells Isolated from Human Glioblastoma Cell Lines

            Ana G. Piña-Medina, Ana M. Hernández-Vega, Nestor F. Díaz, Ismael Mancilla-Herrera, and Ignacio Camacho-Arroyo

4. Reverse Docking for the Identification of Molecular Targets of Anticancer Compounds

            Angel Jonathan Ruiz-Moreno, Alexander Dömling, and Marco Antonio Velasco-Velázquez

5. Mouse Model for Efficient Simultaneous Targeting of Glycolysis, Glutaminolysis, and De Novo Synthesis of Fatty Acids in Colon Cancer

            Alejandro Schcolnik-Cabrera and Alfonso Dueñas-González

Part II: Epigenetic Control of Cancer

6. Developing a Portable Device for the Identification of miRNAs in Fluids

            Alexander Asanov, Alicia Sampieri, and Luis Vaca

7. Methods for the Study of Long Non-Coding RNA in Cancer Cell Signaling

            Yi Feng, Junjie Jiang, Zhongyi Hu, Jiao Yuan, Tianli Zhang, Yutian Pan, Mu Xu, Chunsheng Li, Youyou Zhang, Lin Zhang, and Xiaowen Hu

8. RNA-Sequencing Analysis Pipeline for Prognostic Marker Identification in Cancer

            Sudhanshu Shukla and Seema Khadirnaikar

Part III: Metastasis Promotion

9. Correlation of Circulating Tumor Cell Measurements with 3D Quantitative Tumor Characterization to Predict Clinical Outcomes in Cancer

            Sweet Ping Ng, Clifton David Fuller, and Heath Devin Skinner

10. Extracellular Vesicles and Their Roles in Cancer Progression

            Wen-Hsuan Chang, Richard A. Cerione, and Marc A. Antonyak

11. Analysis of Tumor-Derived Exosomes by Nanoscale Flow Cytometry

            Cynthia Lopez-Pacheco, Andrea Bedoya-Lopez, Roxana Olguín-Alor, and Gloria Soldevila

12. In Vitro Models for Studying Tumor Progression

            Juan Carlos González-Orozco, Saúl Gaona-Domínguez, and Ignacio Camacho-Arroyo

13. Assessment of Cell Cycle in Primitive Chronic Myeloid Leukemia Cells by Flow Cytometry after Co-Culture with Endothelial Cells

            Patricia Torres-Barrera, Mireya Ramírez-Florencio, and Antonieta Chávez-González

Part IV: New Technologies in the Study of Cancer

14. Chimeric Antigen Receptor (CAR) T-Cell Therapy for Cancer: Challenges and Opportunities

            Arimelek Cortés-Hernández, Evelyn Alvarez-Salazar, and Gloria Soldevila

15. Cell-Internalization SELEX of RNA Aptamers as a Starting Point for Prostate Cancer Research

            Mauricio Rodríguez-Dorantes, Sergio Alberto Cortés-Ramírez, Jenie Marian Cruz-Burgos, Juan Pablo Reyes-Grajeda, Alberto Losada-García, Vanessa González-Covarrubias, and Carlos David Cruz-Hernández

16. Generation of Functional Genetic Study Models in Zebrafish Using CRISPR-Cas9

            Francisco Carmona-Aldana, Hober N Nuñez-Martinez, Carlos A Peralta-Alvarez, Gustavo Tapia-Urzua, and Félix Recillas-Targa

17. Developing a Model for a siRNA Delivery System by Cancer Implantation into Zebrafish Circulation

            Yasuhito Shimada and Mai Hazekawa

18. Fabrication of Adhesive Substrate for Incorporating Hydrogels to Investigate the Influence of Stiffness on Cancer Cell Behavior

            Genaro Vázquez-Victorio, Adriana Rodríguez-Hernández, Mariel Cano-Jorge, Ana Ximena Monroy-Romero, Marina Macias-Silva, and Mathieu Hautefeuille

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts